Immunome Research
Open Access
ISSN: 1745-7580
+44-20-4587-4809
ISSN: 1745-7580
+44-20-4587-4809
Yu-Chan Chao
Academia Sinica, Taiwan
Keynote: Immunome Res
Baculoviruses are a group of double-stranded DNA viruses that only infect invertebrates; specifically, lepidopteran insect larvae. They have been approved by many countries as beneficial microbial agents for eradicating insect pests and are also deemed safe for medical applications. Here, we used Autographa californica multiple nucleopolyhedrovirus (AcMNPV), a typical baculovirus as a tool to present the hemagglutinin (HA) of influenza virus onto baculovirus envelope, which we name HA-Bac, as an antigen to immunize mouse for the generation of antibody against hemagglutinin. Anchoring of HA onto the baculovirus envelope required the cytoplasmic domain (CTD) of GP64, a baculovirus envelope protein. HA-Bac can express a GP64-CTD-fused HA on the envelope of baculoviruses and insect cell membranes. Both HA-Bac and cell membraneanchored HA protein were purified from HA-Bac-infected insect cells. Three mice per individual treatment were injected separately with purified HA, as either 0.06 or 30 �?¼g/injection. In addition, a 1�?�?109/shot of HA-Bac (containing 0.06 �?¼g HA) was also applied to injected mice with or without adjuvant. We found that mice injected three times with 0.06 �?¼g/shot purified HA could not generate detectable antibody but all of the 30 �?¼g/shot HA and HA-Bac injected mice (with or without adjuvant) generated strong antibodies against hemagglutinin. These antibodies interacted with HA, as evidence by Western blot analysis and hemagglutination inhibition assays. The results of these experiments show that presentation of hemagglutinin on the envelope of baculovirus can serve as a convenient, easy-to-purify antigen for stimulating antibody production in animals.
Yu-Chan Chao has received his PhD from University of Arkansas and Post-doctoral training at the Cold Spring Harbor Laboratory and Cornell University. He previously served as the Dean of the College of Life Science, National Chung-Hsing University and as Deputy Director at the Institute of Molecular Biology, Academia Sinica, and he is currently a Professor at this latter institute. He has received three Distinguished Research Awards from the Ministry of Science and Technology, Taiwan and was elected as a Distinguished Research Fellow. He has published more than 70 papers and serves as an Editor for several highly regarded international journals.
Email: mbycchao@gate.sinica.edu.tw