Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
+44 1223 790975
ISSN: 2155-9899
+44 1223 790975
Alexander Kwarteng, Alex Debrah, Achim Hoerauf and Sabine Specht
University Hospital Bonn, Germany
Kwame Nkrumah University of Science and Technology, Ghana
Kumasi Centre for Collaborative Research in Tropical Medicine, Ghana
Posters & Accepted Abstracts: J Clin Cell Immunol
Granzyme A and B have been shown to play crucial role in Litomosoides sigmodontis infection, however, their importance in human filariasis remains to be fully characterized. To determine the expression pattern of human granzyme A and B in W. bancrofti-infected individuals as well as endemic normals, FACS intracellular staining was performed and measured with flow cytometer. The frequency of CD4+ T and CD8+ T cells expressing GZMB was significantly higher in W. bancroftiinfected compared to EN. To further establish whether CD4+ T and CD8+ T cells expressing GZMB are infection status depend, group-wise comparisons were performed. Interestingly, increased frequencies of CD4+GZMB+ and CD8+GZMB+ expressing T-cells were observed in the MF+ population compared to MF- and EN. In contrast, when compared to EN, there were no differences in the frequencies of CD4+GZMA+-expressing T in W. bancrofti infected group; however, upon further analysis, GZMA+-producing CD4+ and CD8+ T cells were significantly increased in MF-, but were highly expressed in MF+ and EN. In general the proportion of CD8+GZMA+ expressing T-cells was almost 2 fold of CD4+GZMA+ expressing T-cells. The results demonstrate that individuals with patent infection present increased frequencies of GZMB-producing CD4+ and CD8+ T cells, which may support MF survival or facilitate peripheral circulation given that GZMB has a unique degradation of extracellular matrix, whereas up-regulation of GZMA levels in MF- individuals and EN could promote protection against filarial.
Email: senkwarteng@yahoo.co.uk