Development of the filamentous fungus Trichoderma reesei as an en | 53810
Journal of Fundamentals of Renewable Energy and Applications

Journal of Fundamentals of Renewable Energy and Applications
Open Access

ISSN: 2090-4541

+44 1300 500008

Development of the filamentous fungus Trichoderma reesei as an enzyme production cell factory for biorefineries

Euro-Global Summit and Expo on Biomass

August 08-09, 2016 Birmingham, UK

Markku Saloheimo

VTT Technical Research Centre of Finland Ltd., Finland

Scientific Tracks Abstracts: J Fundam Renewable Energy Appl

Abstract :

In biotechnical production processes of bio-fuels and chemicals from plant biomass, lignocellulose is converted to C5 and C6 sugars by hydrolytic enzymes. They form a major cost factor for the whole process. For this reason a lot of research and development work has been recently invested into making more efficient lignocellulose degradation enzyme cocktails and creating more efficient enzyme production strains. The major production host for bio-refinery enzymes is the fungus Trichoderma reesei that can produce enzyme titers of over 100g/l. Our institute has over 30 years of experience in the molecular, systems and synthetic biology as well as in strain improvement and bioprocess optimization in this production system. We have used a number of different approaches to develop more potent hydrolytic enzyme mixtures and production strains with improved efficiency. For example, we have discovered novel enzymes from other filamentous fungi that can boost up the efficiency of the lignocellulose degradation enzyme mixtures. We have identified and deleted genes encoding major proteases that hamper the production of enzymes in T. reesei, in particular ones from a foreign organism. Furthermore, we have used systems biology approaches to identify new transcription factors regulating enzyme production, and enhanced enzyme productivity by modifying the expression of these factors. The establishment of better enzyme production and hydrolysis technology by these means will be discussed in the presentation.

Biography :

Markku Saloheimo has completed his PhD in 1991 from Helsinki University and Post-doctoral studies in University of Hannover. He is a Research Team Leader at VTT Technical Research Centre. He has published about 90 peer-reviewed papers and is an inventor in 23 patent families.