Journal of Plant Biochemistry & Physiology
Open Access

Detection and verification of SNP related to cotton fiber quality in cotton cellulose synthase gene family

6^th Global Summit on Plant Science

October 29-30, 2018 | Valencia, Spain

Xiangyun Zhang, Jinfa Zhang, Jianhong Zhang, Sujun Zhang, Shuxin Shi, Jie Chen, Zongfu Han, Jiwen Yu, Jianyong Wu, Chaozhu Xing andXihua Li

Institute of Cotton Research, China
New Mexico State University, USA
Huazhong Agricultural University, China
Cotton Research Center, China
ICR - CAAS, China

Posters & Accepted Abstracts: J Plant Biochem Physiol

Abstract :

Cellulose synthases (CesAs) are multi-subunit enzymes associated with the plasma membrane in plants, playing a pivotal role in cellulose production. In this study, the CesA gene coding sequences of four cotton species with sequenced genomes were aligned. The phylogenetic analysis indicated that the CesA gene family in G. arboreum, G. raimondii, G. hirsutum (�??TM- 1�?�) and G. barbadense (�??Xinhai 21�?�) or G. barbadense (�??3-79�?�) could be divided into 6 groups and 15 sub-groups, each group containing 2-5 homologous genes. A total of 544 SNPs were identified in the CesA gene family among the five cotton genomes, including 155 with including amino acid changes. An expression analysis of CesA genes through RNA-seq showed that one to four GhCesA genes were Differentially Expressed (DE) in 0 and 3 DPA ovules between the two BILs (NMGA-062 and NMGA- 105) with different fiber lengths, but no DE gene was identified in 10 DPA fibers. Some SSR markers related to fiber quality were found around CesA Genes. Part of SNPs in the differentially expressed GhCesA genes was checked for fiber quality (in progress). Recent Publications 1. Li F, Fan G, Wang K, Sun F, Yuan Y, Song G, Li Q, Ma Z, Lu C, Zou C, et al. (2014) Genome sequence of the cultivated cotton Gossypium arboreum. Nature genetics 46(6):567-573. 2. Wang K, Wang Z, Li F, Ye W, Wang J, Song G, Yue Z, Cong L, Shang H, Zhu S, et al. (2012) The draft genome of a diploid cotton Gossypium raimondii. Nature Genetics 44(10):1098-1103. 3. Li F, Fan G, Lu C, Xiao G, Zou C, Kohel R J , Ma Z, Shang H, Ma X, Wu J, et al., (2015) Genome sequence of cultivated upland cotton (Gossypium hirsutum TM-1) provides insights into genome evolution. Nature biotechnology 33(5):524- 530. 4. Liu X, Zhao B, Zheng H, Hu Y, Lu G, Yang C, Chen J, Chen J, Chen D, Zhang L, et al., (2015) Gossypium barbadense genome sequence provides insight into the evolution of extra-long staple fiber and specialized metabolites. Scientific reports 5:14139. 5. Li A, Xia T and Xu W (2013) An integrative analysis of four CESA isoforms specific for fiber cellulose production between Gossypium hirsutum and Gossypium barbadense. Planta 237(6):1585�??1597.

Biography :

Xiangyun Zhang, professor, female, born in Hebei province. China. She is engaged in cotton breeding for over 30 years. she has won the National Scientific and Technological Progress Second Prize and China Agricultural Science and Technology Award, and now she obtains State Council special allowance. She and her team has successively presided and finished national or provincial cotton research projects more than 60 items, and nearly 20 high-yielding, disease-resistant and high-quality cotton varieties were bred, such as Ji228, Jiza 1, Jimian 958, Jiyouza69, Ji2000, etc. In addition, she presided over 18 invention patents and achievements. The team led by comrade Xiangyun Zhang laid the foundation of cotton breeding in Hebei province.

E-mail: jimianzhang@qq.com