Detection and genetic characterization of Wolbachia a symbiont ba | 1215
Entomology, Ornithology & Herpetology: Current Research

Entomology, Ornithology & Herpetology: Current Research
Open Access

ISSN: 2161-0983

+44 1478 350008

Detection and genetic characterization of Wolbachia a symbiont bacteria of Culicidae from Parque Ecologico do Tiete, Sao Paulo, Brazil

International Symposia on Entomology

September 04, 2013 Holiday Inn Orlando International Airport, Orlando, FL, USA

Stella Noguera Pereira, F�bio de Almeida and Lincoln Suesdek

Posters: Entomol Ornithol Herpetol

Abstract :

Mosquitoes of the family Culicidae are epidemiologically relevant owing to their vectorial capacity for several human/ animal pathogens. The urban park ?Parque Ecol�gico do Tiet�? (PET) is unique epidemiological scenario in S�o Paulo city (Brazil): Owing to the fact that the park harbours dozens of mosquito species, the thousands of leisure tourists that visit the park weekly are under risk of infection by insect-borne pathogens. In an attempt to enrich the basic knowledge on culicids from PET and to help the development of new strategies to control those mosquitoes, we investigated the presence of Wolbachia in those mosquitoes. The bacteria Wolbachia are vertically-transmitted obligatory endosymbionts of arthropods and nematodes. Owing to its capability of altering the reproduction and other physiological features of its hosts, Wolbachia has been proposed as a ?biological tool? to control Culicidae vectors. We detected the presence of Wolbachia using the amplification and sequencing of the bacterial genes: wsp and 16S rDNA. Seven species (73% of the 216 individual mosquitoes) were infected by Wolbachia , including some abundant species of Aedes and Culex . Phylogenetic tree of the Wolbachia we found (based on 16S) was not congruent to the phylogeny of its respective culicid hosts, revealing that bacteria and hosts have not followed the same evolutionary history. In addition, the high similarity among 16S sequences lead us to hypothesize that Wolbachia transmission in the culicid community of PET is primarily horizontal. If correctly interpreted, this pattern may be suitable for paratransgenesis to reach several species simultaneously.

Biography :

Stella Noguera Pereira graduated in S�o Camilo University (S�o Paulo, Brazil). Present work comprises partial results of her Scientific Iniciation degree which is under development at Butantan Institute (Brazil).