Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Sadjia Lahiani and Moreno Galleni
University of Boumerdès, Algeria
CIP—University of Liège, Belgium
Posters & Accepted Abstracts: J Clin Cell Immunol
Der p 5 is one of the most commonly recognized house dust mite (HDM) allergen in patients suffering from allergic asthma; however, there is no information on its IgG-binding epitopes. In the present study, rabbits were immunized with recombinant Der p 5 allergen and serum samples were obtained. Recognition of linear IgG-epitopes of Der p 5 was determined using synthesized peptides derived from the allergen sequence. The results showed that serum from immunized rabbits recognized three linear epitopes from Der p 5 viz. (28EDKKHDYQNEFDFLLMERIHEQIK43), (37IHEQIKKGELALFYLQEQ55) and (92LMQRKDLDIFEQYNLEMAKKS112). More interesting, we observed that the 92L-S112 amino acid sequence is well recognized by both IgE and IgG antibodies. Der p 5 stimulates the synthesis of specific IgG-antibodies which recognizes common but also novel epitopes compared with IgE-antibodies binding. Indeed, the potential to induce IgG-antibodies can be used to inhibit human-IgE binding to allergens which may be part of the mechanism of action of specific immunotherapy (SIT).
E-mail: sadjialhiani@yahoo.fr