Immunome Research
Open Access
ISSN: 1745-7580
+44-20-4587-4809
ISSN: 1745-7580
+44-20-4587-4809
I E Tothill, Z Altintas, J Pocock and Katy-Anne Thompson
Cranfield University, UK
Public Health England, UK
Posters & Accepted Abstracts: Immunome Res
Comparative investigation for Adenovirus recognition and detection was conducted using natural and plastic antibodies. A direct and sandwich ELISA assays were first developed for adenovirus quantification using natural antibodies on the gold chip of a Surface Plasmon Resonance (SPR) sensor. The assays were optimized and detection limits for the direct and sandwich assays were found to be 0.3 pM and 0.008 pM, respectively. The kinetic data analyses were performed for the different adenovirus recognition methods and cross-reactivity studies were also conducted using MS2 bacteriophage as control virus and an excellent specificity was achieved with all assays types. As an alternative to antibodies the use of molecularly imprinted polymer (MIP) technology for the specific and sensitive recognition of viruses in combination with biosensors was also investigated in this work. Plastic antibodies (MIPs nanoparticles) were designed and synthesized for Adenovirus by employing a novel solid phase production method. MIP receptors were then characterized using dynamic light scattering (DLS) and transmission electron microscopy (TEM) prior to their immobilization on the Surface Plasmon Resonance (SPR) sensor chip as affinity receptor for Adenovirus detection. Two different templates were also imprinted as control MIPs (vancomycin-MIP and the bacteriophage MS2-MIP). The specific recognition of Adenovirus was investigated in the concentration range of 0.01-20 pM and the limit of detection was achieved as 0.02 pM. This work highlights the suitability of plastic antibodies for the detection of viruses and makes comparison with natural antibodies as the current sensing molecules for viruses.
Email: i.tothill@cranfield.ac.uk