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Dablu Lal Gupta, Sanjeev Bhoi and D N Rao
All India Institute of Medical Science, India
Posters & Accepted Abstracts: J Clin Cell Immunol
Introduction: Severe trauma complicated with multiple organ dysfunction syndromes (MODS) is among the leading causes of deaths. Alarmingly, the mortality rate, owing to multiple causes e.g., with or without sepsis is now reported to cross the value of 50%. Objective: The aim of this study is to examine the cause of imbalance in cytokine profile, immune-paralysis (T cell anergy) in trauma hemorrhagic shock (THS) and septic shock patients. Methodology: We have measured the T-cell proliferation assay using dominant antigens of both Gram positive (LTA, 100 ng/ ml) and Gram negative (LPS-100 ng/ml) bacteria and PHA (4 �?¼g/ml) using radioactive thymidine (1H3) assay. At the same time measured the culture supernatant for cytokine levels using Cytokine bead assay (CBA). Results & Conclusion: In the hemorrhagic shock patients, we observed significant (P<0.05) fall T-cell proliferation in comparison to healthy control. Our study also showed patients died due to sepsis/septic shock had low T-cell function and had significantly elevated levels of IL-4, IL-10 and TGF-�?² but very low level of IL-2 and IFN-�?³ in culture supernatant. THS patients who developed sepsis complication had significantly high T-regulatory cells and low Th17 cells. In conclusion, our study showed imbalance in cell mediated immune response and disturbance in Th1/Th2/Th17 and Treg population of T-helper cells and also the shifts towards Th2 and Treg in patients who had developed sepsis and showed poor outcomes.