Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
+44 1300 500008
ISSN: 2167-0412
+44 1300 500008
M Bourkhiss, A Chaouch, M Ouhssine, J Costa and J Paolini
CRMEF, Morocco
Universite Ibn Tofail, Morocco
Universite de Corse, France
Posters & Accepted Abstracts: Med Aromat Plants
With a view to contribute to the evaluation of Moroccan flora through the identification of new potentially interesting compounds at both biological and therapeutic level, we have undertaken a study of essential oils in sawdust of Tetraclinis articulata (Vahl) Masters originating from the Khemisset region (Morocco). The study of the sawdustâ�?�?s essential oil was completed by GC and GC/MS where we identified a total of 22 components and the main five being: �?±-acor�?©nol (20.9%), c�?©drol (17.9%), totarol (8.8%), �?±-c�?©dr�?¨ne (8.7%) and �?²- acor�?©nol (7.4%). This essential oil was fractionated on an open silica column using an eluent of increasing polarity. Five fractions were collected in this way. The fraction (FH) eluted with the pentane comprised sesquiterpene hydrocarbons whereas the other four oxygenated fractions (FO1 to FO4) eluted with a diethyl oxide/pentane mix of increasing polarity were dominated by a diterpene phenol and two sesquiterpene alcohols respectively: totarol in the FO1 (42.4%), �?±-acor�?©nol in the FO2 (34.9%) and FO3 (54.0%), and c�?©drol in the FO4 (58.2%). The sawdustâ�?�?s oil and its chromatographic fractions were tested in vitro against four bacterium: Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Micrococcus luteus, and three fungi: Penicillium parasiticus, Aspergillus niger and Trametes pini. The unfractionated essential oil and oxygenated fractions, particularly FO1, comprising mainly of Totarol were the most active. Therefore, the FO1 fraction would be recommended for optimizing the antimicrobial activity of Tetraclinis articulataâ�?�?s essential oils.