Journal of Drug Metabolism & Toxicology
Open Access
ISSN: 2157-7609
ISSN: 2157-7609
Tadashi Shimamoto, Ashraf M Ahmed, Akito Maruyama and Toshi Shimamoto
Kafrelsheikh University, Egypt
Hiroshima University, Japan
Scientific Tracks Abstracts: J Drug Metab Toxicol
The emergence and dissemination of antimicrobial resistance amongst bacteria is a growing problem for public health worldwide. Large quantities of seafood are consumed in Japan each year; however, little is currently known about the prevalence of integron and antibiotic resistance genes in seafood-associated bacteria in Japan. Therefore, PCR and DNA sequencing were used in the current study to screen and characterize integrons and resistance determinants in 215 Gramnegative bacteria isolated from retail seafood products from Hiroshima Prefecture, Japan. Class 1 integrons were identified in six bacterial isolates (three Aeromonas hydrophila, one Citrobacter freundii, one Enterobacter cloacae, and one Klebsiella oxytoca), and contained gene cassettes encoding resistance to trimethoprim (dfrA12 and dfrA17), aminoglycosides (aadA2), and �?�?�?²-lactams (blaPSE-1). All isolates were negative for class 2 integrons. �?�?�?²-lactamase-encoding genes were identified in seven isolates (four C. freundii and three E. cloacae), including the narrow-spectrum �?�?�?²-lactamase-encoding gene blaTEM-1, and AmpC �?�?�?²-lactamase-encoding genes blaCMY-2, blaCMY-13, and blaCMY-39. blaCMY-39 is a novel gene isolated from a C. freundii strain and encodes resistance to cephamycins and third generation cephalosporins. blaCMY-39 was cloned and expressed in Escherichia coli TG1. Plasmid-mediated quinolone resistance genes, including qnrB2, qnrB6, and qnrS1, were also identified in 10 isolates (six C. freundii, two E. cloacae, one Citrobacter koseri, and one Pantoea spp.). This study highlights the presence of integrons and antimicrobial resistance genes in seafood-associated bacteria in Japan, and indicates that seafood could be a route of transmission of antibiotic-resistant bacteria to humans.
Tadashi Shimamoto has completed his PhD from Okayama University, Japan and Postdoctoral studies from University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School. He is a Professor and a vice dean of Graduate School of Biosphere Science, Hiroshima University. He has published more than 60 papers in reputed journals and has been serving as an Editorial Board Member of Microbiology and Immunology.
Email: tadashis@hiroshima-u.ac.jp