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A H Bandivdekar
National Institute for Research in Reproductive Health, India
Posters-Accepted Abstracts: J Antivir Antiretrovir
Poor proof reading activity of Human Immunodeficiency Virus (HIV) results in to presence of distinct viral variants in different cells and secretions of the same individual. These viral variants may influence the affinity to different host cell CD4 dependent and independent receptors. Sexually HIV has been transmitted by binding to human Mannose Receptor (hMR) on human sperm, vaginal epithelial cells which are devoid of conventional CD4 receptor. HIV binding to hMR induces Matrix Metallo-proteinase 9 which weakens the cell surface and therefore increases the risk for sexual transmission of HIV. Further the localization of hMR was found to be in lower number of vaginal epithelial cells of HIV negative female partner of serodiscordant couples as compared to normal females suggesting the association of hMR in sexual transmission of HIV. Genotypic characterization of C2-V3 region of HIV1 C env gene in PBMCs, sperm, vaginal epithelial cells and cervical cells showed presence of distinct variants in the same individual with variable infectivity and different numbers of N-linked glycosylation sites suggesting variation in co-receptor affinity in different cells of the same individual which may influence disease progression and risk of sexual transmission of HIV. Additionally genotypic characterization of HIV1 gp41 by next generation sequencing showed presence of multiple variants in blood of the same individual. Presence of distinct and multiple HIV variants in PBMCs and urogenital cells may influence the viral affinity to host and immune cells and therefore may affect the transmission, infectivity, pathogenicity and response to ART.