Applied Microbiology: Open Access
Open Access
ISSN: 2471-9315
+44 1300 500008
ISSN: 2471-9315
+44 1300 500008
Regis Sodoyer, Charlotte Mignon, Natacha Mariano and Bettina Werle
Bioaster, France
Posters & Accepted Abstracts: Appli Micro Open Access
During the past decades, the domain of recombinant protein expression has drawn inspiration from molecular engineering techniques associated to fundamental aspects of microbiology and cellular biology. One of the major drivers was the insatiable need for large amounts of biotherapeutic molecules and recombinant vaccines. Molecular biology techniques have, in parallel, gained in sophistication and ease of use, leveraging the creativity associated to the definition of novel expression systems. In addition to industrial needs in terms of productivity and cost effectiveness, safety considerations became increasingly demanding. The consequence was the definition or strengthening of new and logical quality and safety constraints. As a matter of example, the use of alternatives to antibiotic-based selection is promised to introduce new regulatory standards. The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means. We propose a general strategy, based on novel systems validated at both laboratory and pre-industrial scale. Such genetic solutions combine natural plasmid stabilisation and antibiotic-free selection based on post-segregational killing. These novel host/vector systems are completely devoid of antibiotic resistance gene and bring the additional advantage of improving recombinant protein expression and/or plasmid recovery. A dramatic increase in genetic stability and robustness, over high stress fermentation conditions is also often demonstrated. The intrinsic performance of the expression system can be further improved through precise fine tuning of the codon usage, modulating speed and local rhythm of translation. An expected increase in productivity, solubility and global functionality of expressed protein will be discussed.
Email: regis.sodoyer@bioaster.org