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University of Belgrade, Serbia
Posters & Accepted Abstracts: J Phys Chem Biophys
Complex multi-scale interactions among immobilized cell aggregates and the surrounding matrix within the Ca-alginate microbeads at various space scales: (1) at the bio-interface, (2) within the perturbed boundary layers around the cell aggregates, (3) within the microbead parts (consists of a few cell aggregates with perturbed matrix boundary layers and un-perturbed matrix parts) and (4) within the microbead as a whole are considered theoretically based on thermodynamical and rheological approaches. The irreversible nature of the matrix structural changes considered at various space scales is modeled at two time scales i.e., a long time scale (cell growth time), and a short time scale (cell rearrangement time). A comparison of various modeling approaches shed light on the essential mechanism of the structural changes of the matrix sub-system caused by cell rearrangement and growth. The mechanism is connected with energetically perturbed cell states caused by the interactions with the polymer matrix at the bio-interface. These cell states and the rate of their changes induces perturbation of the matrix state at the interface and within the boundary layers around the immobilized cell aggregates. The mechanisms of structural ordering of both sub-systems induce time delaying effects which lead to the anomalous nature of energy transfer and dissipation through the microbeads. The phenomenon could be related to the microenvironmentally restricted cell growth which has a great practical importance in prediction and optimization of various bio-processes such as: (1) mechanically suppressed tumor growth, (2) cell growth and products secretion, (3) artificial organs formation.