Journal of Pollution Effects & Control
Open Access
ISSN: 2375-4397
ISSN: 2375-4397
Sehroon Khan, Sadia Nadir, Zia Ullah Shah, Aamer Ali Shah, Samantha C Karunarathna, Jianchu Xu, Afsar Khan, Shahzad Munir and Fariha Hasan
Kunming Institute of Botany, CAS, China
University of Science and Technology Bannu, Pakistan
Quaid-I-Azam University Islamabad, Pakistan
World Agroforestry Centre, East and Central Asia, China
COMSATS Institute of Information Technology, Pakistan
Yunnan Agriculture University, China
Scientific Tracks Abstracts: J Pollut Eff Cont
The xenobiotic nature and lack of degradability of polymeric materials has resulted in vast levels of environmental pollution and numerous health hazards. Different strategies have been developed and still more research is in progress to reduce the impact of these polymeric materials. This work aimed to isolate and characterize polyester polyurethane (PU) degrading fungi from the soil of a general city waste disposal site in Islamabad, Pakistan. A novel PU degrading fungus was isolated from soil and identified as Aspergillus tubingensis on the basis of colony morphology, macro- and micro-morphology, molecular and phylogenetic analyses. The PU degrading ability of the fungus was tested in three different ways in the presence of 2% glucose: (a) on SDA agar plate, (b) in liquid MSM, and (c) after burial in soil. Our results indicated that this strain of A. tubingensis was capable of degrading PU. Using scanning electron microscopy (SEM), we were able to visually confirm that the mycelium of A. tubingensis colonized the PU material, causing surface degradation and scarring. The formation or breakage of chemical bonds during the biodegradation process of PU was confirmed using Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy. The biodegradation of PU was higher when plate culture method was employed, followed by the liquid culture method and soil burial technique. Notably, after two months in liquid medium, the PU film was totally degraded into smaller pieces. Based on a comprehensive literature search, it can be stated that this is the first report showing A. tubingensis capable of degrading PU. This work provides insight into the role of A. tubingensis towards solving the dilemma of PU wastes through biodegradation.
Sehroon Khan worked in different research areas and fought against chemical pesticides or plastics wastes, which are hazardous for both environment and humans. His research directions are isolation of fungi from soil and other dumping sites that could be utilized for applied side in various aspects to keep the environment safe. For example, isolation of entomopathogenic fungi or plastics biodegrading fungi that could have direct effect on ecosystem conservation. Similarly, isolation of insect toxin proteins from entomopathogenic fungi and identification of their gene sequence and their secretion expression in endophytic bacteria, and the characterization of the engineered strain on biological control. Besides these, he is working on isolation and identification of elicitors proteins from phytopathogenic fungi/oomycetes, and determination of their gene sequence. His research interest also includes the plant immunity mechanism involved in the Systemic Acquired Resistance pathways on molecular level, or developing biological based pesticides to kill the pests through environmentally safe biogenic compounds. More recently, he is involved in isolation, identification, and phylogenetic classification of micro and macro fungi based on morpho-molecular and phylogenetic analyses of ITS rDNA regions.