Reproductive System & Sexual Disorders: Current Research

Reproductive System & Sexual Disorders: Current Research
Open Access

ISSN: 2161-038X

+44 7460731551

Bioanalytical method development and validation of metaxalone in human plasma by LC-MS/MS

2nd International Conference on Reproductive Health and Medicine

June 26-27, 2017 London, UK

Nirav R.Soni

A-One Pharmacy College, India

Scientific Tracks Abstracts: Reprod Syst Sex Disord

Abstract :

A simple, rapid, sensitive, and selective liquid chromatography-tandem mass spectrometry (MS) method was developed and validated for the quantification of Metaxalone, a skeletal muscle relaxant, in human plasma using Metaxalone –D6 as Internal Standard (IS). Following Liquid-Liquid Extraction (LLE) , the analytes were separated using an isocratic mobile phase on a reverse phase C18 column (Chromatopak peerless basic 50× 4.6mm× 3.0μm ) and analyzed by MS in the multiple reaction monitoring mode using the respective [M+H]+ ions, m/z 222.14 / 160.98 for Metaxalone and m/z 228.25 /167.02 for the IS. The assay exhibited a linear dynamic range of 25.19 - 2521.313 ng/mL for metaxalone in human plasma. The goodness of fit was consistently greater than 0.98 during the course of validation. The range of accuracy and precision of the back-calculated concentrations of the standard curve points was from 94.1 % to 104.4 % and 0.3 % to 5.6 % for metaxalone A run time of 2.0 min for each sample and injection volume is 5 μl. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability and bioequivalence (BA-BE) studies.

Biography :

Nirav has completed his M.Pharm at the age of 23 years from Gujarat Technological University (GTU) and he has taken Post-Graduate Diploma Intellectual Property Rights (PGDIPR). He is the Ass.professor in A-One Pharmacy College, India. He got “Best Teaching Award” and he has achieved award of “Best-Student of the year” for the year 2013. He has published research , review articles and letter to the editors in reputed journals. He has been serving as an editorial board member as well as reviewers of repute. Recently, He got "National Award"- PEARL Foundation “Best Assistant Professor Award in Pharmacy” for the year 2016. He cracked GPAT (Graduate Pharmacy Aptitude Test-2013, AICTE) and got 12,400 INR stipends per month up to two years during post-graduate course from the Indian government. Research Experience in Bioanalytical department and published thesis. Also, He has published Books with ISBN.

Email: [email protected]