Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
ISSN: 2167-0412
Sunita Shailajan and Yugandhara Patil
Ramnarain Ruia Autonomous College, India
Posters & Accepted Abstracts: Med Aromat Plants
The prevalence of PCOS has shown alarming rise. Modern treatments available are oral contraceptives, clomiphene citrate, etc. which involve lot of complications and show adverse effects including obesity and sterility. Other associated symptoms also impose a critical health concern. Hence, alternative therapy for the management of PCOS is urgently required. Traditional formulations that show steady effect with minimal side effects are prescribed against PCOS. One such formulation is Pushyanuga churna (PC), an Ayurvedic formulation described in AFI against various female reproductive disorders. Although, PC is available OTC, scientific data on standardization and QC is unavailable which leads to undesired quality and variation. Hence, standardization is of critical importance. In the current study, PC was prepared as per AFI and QC parameters which include phytochemical and physicochemical analysis were established. Phytochemical fingerprints were developed for inhouse formulation using HPTLC. Further, HPTLC methods were developed and validated as per ICH guidelines for estimation of six biomarkers viz. betasitosterol, lupeol, ursolic acid, quercetin, berberine and glycyrrhizic acid which are reported in managing PCOS. Safety was established following OECD guidelines no.420 in wistar rats. Standardized in-house formulation was evaluated for its efficacy in letrozole induced PCOS in rats and its results were compared with modern control clomiphene citrate in terms of biochemical parameters and histopathology. The results confirmed efficacy of PC in treating PCOS and can be used as baseline for formulating PC as a more patient compliant delivery system. This data can be adapted to lay down new pharmacopoeial standards for batch-to-batch consistency.
E-mail: patil.yugandhara@yahoo.co.in