Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
ISSN: 2167-0412
Bushra Ahmad
Government College Women University, Pakistan
Posters & Accepted Abstracts: Medicinal Plants
Free radicals e.g., reactive oxygen/nitrogen species are key hazards for the living cells because biochemical changes provoked by ROS led to the onset of number of chronic diseases such as cancer, aging, diabetes, arthritis and inflammation. Carissa opaca is traditionally used in Pakistan for the treatment of liver ailments e.g., jaundice and hepatitis. This research project was designed to isolate the active compounds and to further demonstrate the antioxidant proficiency. Prunin, a flavanone glycoside, was isolated first time from C. opaca leaves following repeated column chromatography and thin layer chromatography. Structural elucidation was done using various 1D and 2D NMR techniques. Bioassay-guided fraction scheme was adopted to get pure compound. Antioxidant capacity was monitored through various in vitro radical based screenings e.g., DPPH, ABTS, OH, phosphomolybdate, superoxide anion and H2O2 radicals. Prunin has demonstrated appreciable quenching proficiencies with IC50 values against DPPH (22.1�?±1.07 �?µg/ml), ABTS (29.6�?±0.81 �?µg/ml), phosphomolybdate (29.6�?±0.37 �?µg/ml), superoxide anion (11.1�?±0.49 �?µg/ml) and OH (17.4�?±0.16 �?µg/ml) and H2O2 (11.2�?±0.47 �?µg/ml). IC50 values of Prunin were competing standards. Prunin exhibited strong reducing ability with absorbance of 2.78�?±0.13 at 100 �?µg/ml to that of standard (3.24�?±0.11) ascorbic acid at the same concentration. Production of lipid peroxides was estimated in terms of thiobarbituric acid reactive substances (TBARS) and prunin was found very efficient against TBARS (97 �?µg/ml) to that of ascorbic acid (95 �?µg/ml) during the in vitro system. We concluded from this research project that Prunin was a very active antiradical and potent antioxidant flavonoid that can be very helpful for the development of pharmaceutical drugs.