Journal of Clinical & Experimental Dermatology Research
Open Access
ISSN: 2155-9554
+44 1478 350008
ISSN: 2155-9554
+44 1478 350008
Toni Febriyanto, Susan Simanjaya, Juan Adrian, Mohammad Galih Pratama, Eka Nur Fitriyani and Dewajani Purnomosari
Universitas Gadjah Mada, Indonesia
Posters & Accepted Abstracts: J Clin Exp Dermatol Res
Background: Ultraviolet (UV) exposure causes photoaging by inducing the expression of Matrix Metalloproteinase-1 (MMP- 1) expression. Snail Achatina fulica is one of the most extensively studied snails due to its economic, ecological and medical importance. Moreover, the slime is well-known for its wound-healing and anti-bacterial properties. Objective: The research is aimed to investigate whether snail slime of Achatina fulica exhibits anti-photoaging properties by inhibiting the expression of MMP-1. Methodology: 50 snails were stimulated by a 9V electricity source for the extraction of slime. The extracted slime was then converted into powder form by the freeze-drying process. The slime powder was dissolved in Dimethyl Sulfoxide (DMSO) to desired concentration. At the end of the experiment, the MMP-1 expression in different fibroblast groups was assessed by qPCR. Results: Administration of snail slime has proven to lower MMP-1 expression compared to the positive control group without snail slime. However, administration of snail slime at various concentrations did not significantly produce different results. MMP-1 expression level is slightly lower in slime group compared to the Ellicina cream group at the concentration of 25 and 50 μg/mL. Administration of Ellicina cream at the concentration of 100 μg/mL has shown to lower MMP-1 expression compared to slime group at any concentration. Conclusion: Snail slime of Achatina fulica reduces MMP-1 expression level on UVB-induced fibroblast culture. The potency in inhibiting MMP-1 expression level between Achatina fulica slime and Ellicina cream is not much different at certain concentrations.
Toni Febriyanto is pursuing his medical degree at Universitas Gadjah Mada, Yogyakarta, Indonesia. He is interested in biomedical and clinical research.
E-mail: toni.tfeb@gmail.com