Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
+44 1223 790975
ISSN: 2155-9899
+44 1223 790975
S M P Firoozabadi, M Mansourian and Z M Hassan
Tarbiat Modares University, Iran
Posters & Accepted Abstracts: J Clin Cell Immunol
Statement of the Problem: Electrochemotherapy as a powerful treatment of solid tumors, using electric pulse permeabilised the cells to chemotherapy drug reversibly and in addition to destruction of primary tumor, it is capable of triggering immune antitumor responses and of thus facilitating deletion of tumor cells at metastatic tumor sites. However, the efficacy of such a treatment could be influenced by confounders like mobile phones waves through phenomena such as adaptive response resulted in decreasing of genotoxic agents effects. This study examines alteration in the antitumor effectiveness of electrochemotherapy treatment when tumor cells were pre-exposed to 900 MHz modulated by 217Hz or 217 Hz fields similar to those generated by mobile phones. Methodology: The 4T-1 cells were exposed to ELF magnetic fields at 93, 120 or 159 �?¼T intensities generated by Helmholtz coils or the radiofrequency (RF) signals modulated by rectangular pulses with a repetition frequency of 217 Hz (pulse width 0.576 ms) at 17, 162 or 349 �?¼W/cm2 power densities generated by GSM900 simulator. Then, the cells were treated by different protocols of electrochemotherapy. After 24 hours, the cell viability was evaluated by MTT assay. Findings: The data indicated that although it was not observed any alteration in cell viability as a result of ELF magnetic fields or RF radiations exposure alone, exposure of cells to ELF fields at some flux density before electrochemotherapy to different protocols increases cell viability and thus decrease treatment efficacy. Also, the similar results to that of ELF magnetic fields on treatment efficacy were observed for modulated radiofrequency signals. Conclusion & Significance: Based on the results of this in vitro study, fields emitted by mobile phones can develop an adaptive response for some electrochemotherapy protocols. Therefore, these results should be extended to in vivo studies and be investigated the other mechanism of electrochemotherapy like stimulation of immune response influenced by mobile phone radiations.
Email: pourmir@modares.ac.ir