Journal of Drug Metabolism & Toxicology
Open Access
ISSN: 2157-7609
+44-77-2385-9429
ISSN: 2157-7609
+44-77-2385-9429
Awadhesh Kumar Rai
University of Allahabad, India
Posters-Accepted Abstracts: J Drug Metab Toxicol
Laser Induced Breakdown Spectroscopy (LIBS) is an appealing technique for the detection and identification of all class of materials because of its intrinsic capability for minimally destructive, in situ, real-time detection and analysis of a broad range of chemical species. Because of such remarkable feature this technique has now been widely used to study various materials including plant materials. It is basically an atomic emission spectroscopy technique which utilizes a highly energetic laser pulse as the excitation source. My goal in the present talk is to demonstrate the capability of LIBS to study the presence and deposition pattern of major and minor elements in different parts of the plant sample (wheat plant). Point detection capability of LIBS has been used for the determination of different elements in root, stem and leaf of the plant samples. The results obtained from the LIBS technique are compared with the results obtained by other conventional technique. Chromium is one of the most toxic heavy metal and it found on the Earth in several oxidation states in which Cr (III) and Cr (VI) are most stable oxidation states. Cr (VI) is more toxic to living organisms because of its negatively charged ion which has capability to cross cellular membranes easily by means of sulfate ionic channels and react with the intracellular components. Thus chromium adversely affects the internal and external integrity of plants like growth, water balance, yields, antioxidant metabolism, pigments, cellular arrangements etc. In the present talk I will describe the use of LIBS technique for in situ point-to-point monitoring/detection of Cr in various parts of the plants after exogenous application of Cr. The addition of Si to the growth medium alleviated the effects of Cr (VI) and I will also demonstrate that how the LIBS is useful to elucidate Si-mediated alleviation of Cr(VI) toxicity by using LIBS spectra as a fingerprint of the elemental compositions in wheat seedlings, which showed a reduction in Cr accumulation following Si addition.