Journal of Clinical Chemistry and Laboratory Medicine
Open Access

The Significance of NanoVelcro Chip in Cancer Detection

Richard Meon Gitimu^{* *}Correspondence: Richard Meon Gitimu, Department of Biochemistry and Biotechnology, Kenyatta University, Nairobi, Kenya, Email:

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Introduction

Nanovelcro chip is a significant invention for the tracing of circulating tumor cells. The framework of the chip for insertion and location is evident of its use and application.

Nanovelcro chip

The NanoVelco chip is of three parts: 1) a serpentine chaotic blender chip prepared by PDMS, 2) A designed SiNW substrate with anti-EpCAM coating which has high affinity, and 3) an aligned PDMS blender chip with SiNW substrate.The PDMS blender chips were created utilizing a standard delicate lithography strategy. To limit framework error brought by irregularity of PDMS blender chips, a silicon mold with an ideal arrangement was made by dry carving/etching, and outlined in a metal holder. Well-mixed PDMS was poured onto this form and released in a oven at 80°C. After 48 h of heating, the restored PDMS layer was stripped off and poked with two holes at the two closures of the channel for tubing connection. Reliable and consistent channel construction and PDMS thickness were accomplished using this strategy [1]. Over the previous years, clinical exploration in the area of nanotechnology has gained huge progressed in diminishing the expenses of CTC portrayal. The objective of customized care is one-step closer with the arising progresses in oncology. Realizing that the nanoscale tissue microenvironment (especially the cellular membrane and extracellular network) can assist with interceding cell behaviour, the Tseng Lab at UCLA dispatched the nanosubstrate microfluidic stage (i.e., silicon nanowire substrate, SiNS for capturing CTCs, considered the "NanoVelcro" assay. This novel methodology used capture specialist covered nanosubstrates to immobilize CTCs with high proficiency, looking like the functioning system of Velcro. Similarly that two texture portions of a Velcro fastener join firmly together, the NanoVelcro cell- affinity substrates communicate with CTCs to shape solid binding [2].

Advantages

In examination with other CTC capturing advancements, the NanoVelcro Chip enjoys several prominent advantages. In the first place, the small size of NanoVelcro Chip joined with its SiNW substrate takes into consideration both transiently fast cell catch and quick imaging. The NanoVelcro Chip catches CTCs onto an exceptionally tight central plane considering a straightforward 2D sweep to cover every one of the caught occasions. This significantly works with the rest of preparation including gating of the cell size, DAPI, CD45-FITC and CK-PE intensity to distinguish possible CTCs. The candidate CTCs are manually affirmed and all debris like cells are killed. Furthermore, the separation and count convention is genuinely basic and easy to understand and can be effortlessly moved to any lab with a fundamental example of taking care with procedures [3].

Limitations

The NanoVelcro Chip lucidly has a robust capture productivity and reliable consistency. However it is not yet shown that the NanoVelcro Chip can be utilized to acquire pivotal atomic data from capture CTCs. Our future endeavors will be given to portraying the atomic marks of CTCs, including genomic, transcriptional and proteomic investigation. Since the caught CTCs are alive, these cells might actually be extended ex vivo for additional sub-atomic cross examination. The more prominent test of acquiring organically practical CTCs that can be utilized for ex vivo culture. This would consider describing the social attributes. Such a cycle would make another method for assessing drug affectability and opposition in individual patients. Utilizing a quantitative immunocytochemistry approach was recently described [4].

Conclusion

The utilization of nanotechnology in clinical oncology has shown a promising future to address a heap of neglected requirements. As the information and comprehension of malignant growth keeps on advancing, cancer biologists and researchers hope to describe the unique science of the illness. In such an advancing organic climate, oncologists are now acquainted with taking care of handling variety of information. Examination of CTCs and other circulating biomarkers has shown guarantee for exploring the unique science in the individual patient. From the molecular organic ideas of DNA, RNA and proteins, it is apparent that there is a lot of difference in the personalities of patients and tumors. Using a noninvasive way to investigate these distinctions can assist with associating the lab and the center. Early triumphs in this field have filled an inspiration for forwarded work to seek after interdisciplinary exploration in translational medication.

References

1. Wang S, Liu K, Liu J, Yu ZT, Xu X, Zhao L, et al. Highly efficient capture of circulating tumor cells by using nanostructured silicon substrates with integrated chaotic micromixers. Angew Chem. 2011;123(13):3140-3144.
2. Wang S, Wang H, Jiao J, Chen KJ, Owens GE, Kamei KI, et al. Three‐dimensional nanostructured substrates toward efficient capture of circulating tumor cells. Angew Chem Int Ed.2009;48(47):8970-8973.
3. Stott SL, Lee RJ, Nagrath S, Yu M, Miyamoto DT, Ulkus L, et al. Isolation and characterization of circulating tumor cells from patients with localized and metastatic prostate cancer. Sci Transl Med.2010;2(25):25ra23
4. Lu YT, Zhao L, Shen Q, Garcia MA, Wu D, Hou S, et al. NanoVelcro Chip for CTC enumeration in prostate cancer patients. Methods. 2013;64(2):144-152.