Severe Thromboembolism and Systemic Lupus Erythematosus Developin
Journal of Hematology & Thromboembolic Diseases

Journal of Hematology & Thromboembolic Diseases
Open Access

ISSN: 2329-8790

+44 1478 350008

Case Report - (2014) Volume 2, Issue 4

Severe Thromboembolism and Systemic Lupus Erythematosus Developing after Ovarian Hyperstimulation in a Persistent Carrier of Antiphospholipid Antibodies

Grandone Elvira1, Orefice Giuseppe2, Jose Delgado Alves3 and Ames Paul RJ4*
1Department of Atherosclerosis & Thrombosis Unit, I.R.C.C.S. "Casa Sollievo della Sofferenza", S. Giovanni Rotondo, Italy
2Department of Neurological Sciences, Università degli Studi di Napoli Federico II, Naples, Italy
3Internal Medicine Department, New University of Lisbon, Lisbon, Portugal
4Queen Mary University Hospital London and St George’s Hospital NHS Trust, London, UK
*Corresponding Author: Ames Paul RJ, Queen Mary University Hospital London and St George’s Hospital NHS Trust, London, UK, Tel: +44 -0-20 8223 Email:


Ovarian hyperstimulation (OH) is not a contraindication in selected women with antiphospholipid syndrome and/or systemic lupus erythematosus (SLE). A young woman who was persistently positive antiphospholipid antibodies, low protein free S and homozygous for the paraoxonase G192A mutation underwent three courses of OH; after the fourth course she developed severe thromboembolism and clinical features of SLE despite the use of a gonadotropin-releasing hormone agonist alongside aspirin that should have minimised the risk of OH syndrome. We provide a pathogenetic interpretation of these events in the light of the patient’s investigations.

Keywords: Ovarian hyperstimulation; Antiphospholipid antibodies; Systemic lupus erythematosus


Primary antiphospholipid syndrome (PAPS) is characterised by arterial and or venous thrombosis in the presence and persistence of antiphospholipid antibodies (aPL) in patients without any underlying autoimmune disorder [1]. A large series demonstrated that 23% of 165 PAPS patients progressed towards a well-defined systemic autoimmune disease during a mean 9.7 years of follow-up in the absence of any precipitating factors [2]. Ovarian hyperstimulation (OH) for ovulation induction does not represent a major concern in selected women with APS and/or systemic lupus erythematosus (SLE) provided patients prone to potential serious complications are excluded by a preliminary risk assessment [3]. A young who was persistently positive for aPL developed SLE and severe vascular events after OH allowing us to discuss some pathogenetic aspects inherent to her case.

Case presentation

In February 1996 a 22 year-old girl presented to neurology for right hemichorea of three days duration: a prolonged activated partial thromboplastin time (aPTT) was confirmed later as a lupus anticoagulant (LA). Her baseline and follow-up investigations are shown in Table 1. She had elevated IgG anticardiolipin antibody (aCL), strong LA, positive antinuclear antibody (ANA) (1:320), normal liver and kidney function tests, but no signs or symptoms of any autoimmune systemic disorder. Her free protein S was low (52%), much lower than the average of our other PAPS patients with acquired free protein S deficiency (84 ± 5.2%) [4]; we could not rule out hereditary free protein S deficiency as she had been adopted and did not know her parents. Amongst others she was also homozygous mutated for the paraoxonase (PON) G192A transition with a PON activity of 126 U/L compared to an average of 292 ± 86 U/L from 20 normal subjects.

  Feb Sept Sept Oct Sept May Sept Apr Feb Feb  
1996 1996 1997 1998 1999 2000 2001 2002 2004 2005
KCTr 2.09 3.79 4 4.9 2.25 3.95 3.21 4.04 3.86 7.36 >1.30
aPTTr 2.37 4.05 3.89 3.41 4.06 3.46 4.05 3.8 3.48 3.24 >1.20
DRVVTr 1.46 1.9 1.97 2.11 2.13 2.16 2.33 2.18 2.09 2.76 >1.18
IgGaCL  (GPL) 124 113 326 529 363 502 410 241 294 489 <20
IgMaCL (MPL) 12 23 25 46 16 31 32 50 14 89 <20
IgGb2GPI (IU/ml)             367   344   <12
FNG (mg/dl) 240 345 335   390 362 481 510 362 480 200-380
PS free (%) 52 49                  
PS total  (%) 90 80                  
PC (%) 118                    
vWF (IU/ml) 103 97 100   98   94        
PAI (mg/dl) 42 36         72        
HC (mmol/L) 16.9 12.6     12   18 10 14 13 <11.9
ANA 0.26389     0.26389           0.48611  
Anti ds DNA 64     86           128  
C3 (mg/dl) 102     112           48 76-136
C4 (mg/dl) 89     98           46 14-76
      OH OH   OH       OH  
      ASA ASA   ASA       ASA  

Table 1: Base line and Follow up Investigations KCT: Kaolin Clotting Time; aPTT: Activated Partial Thromboplastin Time; DRVVT: Dilute Russel Viper Venom Time; r: Ratio; aCL: Anti Cardiolipin; ß2GPI: Beta-2-Glycoprotein-I; FNG: Fibrinogen; PS: protein S; PC: protein C; vWF: von Willebrand factor; PAI: plasminogen activator Inhibitor; HC: Homocysteine; ANA: Antinuclear Antibodies; OH: Ovarian Hyperstimulation; ASA: Acetylsalicylic Acid.

A cerebral MRI revealed pin like lesions in the white matter of the basal ganglia bilaterally. Her hemichorea settled spontaneously but represented 12 months later only to disappear after 1 week of treatment with oral prednisone. A repeat cerebral MRI was unchanged compared to the previous one. She was started aspirin 100 mg daily. Two years later she married the son of her adoptive parents, but did she not conceive for almost 6 years during which concerns about fertility were raised. Her karyotype, infertility and hormonal tests, infection screen, assessment of tubal damage and of uterine abnormalities were normal. Her partner also had a normal karyotype but a degree of sperm hypomotility. As she moved to a different town, she was seen only once from February 2004: on that occasion and as part of the annual review of PAPS patients she underwent a doppler ultrasound of the carotid arteries and an echocardiogram: the former was normal but the latter showed a bicuspid aortic valve. From August 1997 to May 2000 she underwent three 7 day courses of ovulation hyper-stimulation with Gn-RH agonist combined with a Gn-RH antagonist, all covered by aspirin 100 mg that were uneventful but not followed by conception.

Two weeks after the fourth OH in 2005, she was admitted as an emergency for marked tachypnea, chest pain, jaundice, rash, ankle oedema, and joint pains. Investigations revealed Hb 7.6 g/dl, increased indirect bilirubin and LDH, positive direct antiglobulin test and undetectable haptoglobins in keeping with autoimmune haemolytic anaemia, platelets were 73x109/L, albumin was decreased at 22 g/l, creatinine was raised at 255 mmol/L; there was proteinuria (2.8 g/24 hours) with slight haematuria. ANA and anti-ds-DNA were positive while C3 and C4 were low.

An abdominal and pelvic CT scan revealed bilateral pleural effusions and a left renal infarction, whereas a CT pulmonary angiogram revealed bilateral emboli; a transthoracic echocardiogram revealed severe aortic regurgitation that required an urgent replacement. She received high dose steroids, 6 pulses of cyclophoshamide 500 mg every three days followed by maintenance azathioprine, low molecular weight heparin in treatment dose (enoxaparin 120 mg s/c daily for 7 days) embricated with warfarin at a target INR range 2.0-3.0. After these events the patient moved out of the region but underwent an occasional review in April 2008: at that time she was still on warfarin and on azathioprine, her haemolytic anaemia had settled and the proteinuria ranged between 0.8-1.2 g/24 hours.


The major risk of ovarian induction is OH syndrome that encompasses ovarian oedema, electrolyte imbalance, and diffuse capillary leak with resulting pleural effusions and ascites, and, at times, hypotension, hypercoagulability and thrombosis [5]. Vascular occlusions following OH have not been described in PAPS [6], but for a case of cerebral infarction developed in a PAPS patient with an obstetric history [7]; on the other hand intra-cardiac occlusion [8], ischaemic strokes [9,10] and catastrophic APS [11] have been described in carriers of aPL who had never suffered thrombosis. With regards to the development of SLE after PAPS, this has been detailed in two reviews over the last 12 years but OH was not involved in any of the progressions from PAPS towards SLE [12,13].

The thrombogenic potential of OH is unclear in isolated carriers of aPL and in PAPS patients: one possibility is a further increase in aPL titre after OH. Upon treatment with oestrogens, gonadectomized or intact male and female non-autoimmune C57BL/6 mice generate aPL that may persist for months after the oestrogen exposure [14,15]. In addition, an end-metabolite of 17-β-estradiol may induce the conversion B lymphocytes into immunoglobulin secreting plasma cells [16]. This would be in keeping with the finding of increased aPL titres in women receiving more than one course of OH for in vitro fertilization as well as explaining the aPL increase in our patient after OH [17]. The latter study also showed that the elevated PL titre was negatively related to the antioxidant capacity of PON [17]. PON accounts for most of the antioxidant of properties of HDL against LDL oxidation [18] and its activity is known to be decreased in PAPS [19].

Interestingly, pregnancy negative infertile woman who never conceived had lower PON activity than women who became pregnant but OH increased PON activity promoting a favorable environment for implantation [20]. Our patient exhibited a 57% reduction of PON activity partly due to her homozygous PON G192A mutation and partly to her aPL favoring infertility and her PON activity could not rise after OH because of the same reasons.

It is well established that aPL are associated with an enhanced oxidative state [21] and any decrease of antioxidant capacity may favour oxidative stress that may further enhance thrombin generation with consequent vascular occlusions [22]. The need for thromboprophylaxis before OH had been stressed [23] but in our case aspirin cover proved insufficient and with hindsight enoxaparin should have been considered.


We have reported upon a persistent carrier of aPL who developed extensive vascular disease and SLE after OH despite aspirin cover. OH developed after the GnRH agonist/antagonist regime that should minimize the risk of OH syndrome at the expense of a lower 6% delivery rate compared to human chorionic gonadotropin [24]. The distinction between OH syndrome and APS remains semantic as thrombosis is a clinical feature of both, but a review of possible thrombogenic pathways should have led to maximising thromboprophylaxis with low molecular weight heparin for three months as suggested by the American College of Chest Physicians after resolution of clinical OHS syndrome (Grade-2C recommendation) [25]. Although we do our best as physicians to help unfertile women to conceive and carry on with their pregnancy, careful risk assessment should be applied in cases like ours to avoid disastrous outcomes after OH.


Written informed consent was obtained from the patient for publication of this Case report and a copy of the written consent is available for review by the Editor-in-Chief of this journal.

Competing interests

The authors declare no competing interests


  1. Miyakis S, Lockshin MD, Atsumi T, Branch DW, Brey RL, et al. (2006) International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS). J ThrombHaemost 4: 295-306.
  2. Veres K, Szodoray P, Szekanecz Z, Lakos G, Kiss E, et al. (2010) Clinical and immunoserological characteristics of the transition from primary to overlap antiphospholipid syndrome. Lupus 19: 1520-1526.
  3. Bellver J, Pellicer A (2009) Ovarian stimulation for ovulation induction and in vitro fertilization in patients with systemic lupus erythematosus and antiphospholipid syndrome. FertilSteril 92: 1803-1810.
  4. Ames PR, Tommasino C, Iannaccone L, Brillante M, Cimino R, et al. (1996) Coagulation activation and fibrinolytic imbalance in subjects with idiopathic antiphospholipid antibodies--a crucial role for acquired free protein S deficiency. ThrombHaemost 76: 190-194.
  5. Beerendonk CC, van Dop PA, Braat DD, Merkus JM (1998) Ovarian hyperstimulation syndrome: facts and fallacies. ObstetGynecolSurv 53: 439-449.
  6. Guballa N, Sammaritano L, Schwartzman S, Buyon J, Lockshin MD (2000) Ovulation induction and in vitro fertilization in systemic lupus erythematosus and antiphospholipid syndrome. Arthritis Rheum 43: 550-556.
  7. Koo EJ, Rha JH, Lee BI, Kim MO, Ha CK (2002) A case of cerebral infarct in combined antiphospholipid antibody and ovarian hyperstimulation syndrome. J Korean Med Sci 17: 574-576.
  8. Andrejevic S, Bonaci-Nikolic B, Bukilica M, Macut D, Miljic P, et al. (2002) Intracardiac thrombosis and fever possibly triggered by ovulation induction in a patient with antiphospholipid antibodies. Scand J Rheumatol 31: 249-251.
  9. Grandone E, Vecchione G, Colaizzo D, Cappucci F, Margaglione M (2004). Homocysteine and antiphospholipid antibodies in a woman undergoing ovarian follicular stimulation: prospective clinical and laboratory evaluation. Am J ObstetGynecol 191: 370- 371
  10. Girolami A, Scandellari R, Tezza F, Paternoster D, Girolami B (2007) Arterial thrombosis in young women after ovarian stimulation: case report and review of the literature. J Thromb Thrombolysis 24: 169-174.
  11. Giner V, Oltra MR, Esteban MJ, García-Fuster MJ, Salvador A, et al. (2007) Catastrophic antiphospholipid syndrome related to severe ovarian hyperstimulation. ClinRheumatol 26: 991-993.
  12. Blanco Y, Ramos-Casals M, García-Carrasco M, Cervera R, Font J, et al. (1999). Primary antiphospholipid syndrome evolving into systemic lupus erythematosus: a report of 3 new cases and a review of the literature. Rev ClinEsp 199: 586-588
  13. Gómez-Puerta JA, Martín H, Amigo MC, Aguirre MA, Camps MT, et al. (2005) Long-term follow-up in 128 patients with primary antiphospholipid syndrome: do they develop lupus? Medicine (Baltimore) 84: 225-230.
  14. Verthelyi D, Ansar Ahmed S (1997) Characterization of estrogen-induced autoantibodies to cardiolipin in non-autoimmune mice. J Autoimmun 10: 115-125.
  15. Verthelyi DI, Ahmed SA (1998) Estrogen increases the number of plasma cells and enhances their autoantibody production in nonautoimmune C57BL/6 mice. Cell Immunol 189: 125-134.
  16. Cayer MP, Drouin M, Proulx M, Jung D (2010) 2-Methoxyestradiol induce the conversion of human peripheral blood memory B lymphocytes into plasma cells. J Immunol Methods 355: 29-39.
  17. Delgado Alves J, Radway-Bright EL, Lee S, Grima B, Hothersall J, et al. (2005) Antiphospholipid antibodies are induced by in vitro fertilization and correlate with paraoxonase activity and total antioxidant capacity of plasma in infertile women. Lupus 14: 373-380
  18. Mackness MI, Arrol S, Abbott C, Durrington PN (1993). Protection of low-density lipoprotein against oxidative modification by high-density lipoprotein associated paraoxonase. Atherosclerosis 104: 129-135
  19. Delgado Alves J, Ames PRJ, Donohue S, Norouz-Zadeh J, Isenberg DA (2002). Antibodies towards high density lipoprotein, apo-A-I and cardiolipin inversely correlate to para-oxonase activity in systemic lupus erythematosus and primary antiphospholipid syndrome. Possible atherogenic mechanisms. Arthritis Rheum 46: 2686-2694
  20. Younis A, Clower C, Nelsen D, Butler W, Carvalho A et al (2012). The relationship between pregnancy and oxidative stress markers on patients undergoing ovarian stimulations. J Assist Reprod Genet 29: 1083-1089
  21. Ames PR, Nourooz-Zadeh J, Tommasino C, Alves J, Brancaccio V, et al. (1998) Oxidative stress in primary antiphospholipid syndrome. ThrombHaemost 79: 447-449.
  22. Rota S, McWilliam NA, Baglin TP, Byrne CD (1998) Atherogenic lipoproteins support assembly of the prothrombinase complex and thrombin generation: modulation by oxidation and vitamin E. Blood 91: 508-515.
  23. Várnagy A, Koppan M, Manfai Z, Busznyak C, Bodis J (2008) Low-dose aspirin for prophylaxis of ovarian hyperstimulation syndrome. FertilSteril 89: 1035-1036.
  24. Humaidan P, Kol S, Papanikolaou EG; Copenhagen GnRH Agonist Triggering Workshop Group (2011) GnRH agonist for triggering of final oocyte maturation: time for a change of practice? Hum Reprod Update 17: 510-524.
  25. Guyatt GH, Akl EA, Crowther M, Gutterman DD, Schuünemann HJ for the American College of Chest Physicians Antithrombotic Therapy and Prevention of Thrombosis Panel (2102). Antithrombotic Therapy and Prevention of Thrombosis, 9th ed: American College of Chest Physicians Evidence-Based Clinical Practice Guidelines. Chest 141: (Suppl): 7S-47S.
Citation: Elvira G, Giuseppe O, Alves JD, Ames Paul RJ (2014) Severe Thromboembolism and Systemic Lupus Erythematosus Developing after Ovarian Hyperstimulation in a Persistent Carrier of Antiphospholipid Antibodies. J Hematol Thrombo Dis 2:150.

Copyright: © 2014, Ames Paul RJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.