ISSN: ISSN: 2329-6674
McGill University, Canada
Farhad Karbassi received his PhD in Biophysics from University of Tehran. During his PhD program, he was granted to work on a part of his research at National Research Council of Canada / Biotechnology Research Institute (NRC/BRI). He then moved to Canada to work as a postdoctoral fellow. Currently he holds a research associate position at McGill University.
Beside protein engineering, expression, purification and characterization, as well as protein-protein interaction and aggregation studies, Dr. Karbassi has been involved in enzymology. His main interests are enzyme inhibition, stabilization and suicide inactivation. He has extracted different enzymes from various organisms such as bacteria, mushroom, C. elegans, and human; and studied their functions.
Enzyme assays for alpha-amylase, tyrosinase, superoxide dismutase, enolase (bacterial and mammalian), hexokinase, lactate dehydrogenase; Ligand binding studies on alpha-amylase; Transformation, expression and purification of recombinant protein domains, seven types of superoxide dismutase mutants and two types of octameric enolase; Finding optimum conditions for protein purification; Enzyme inhibition and Suicide inactivation studies; Enzyme modification; Determination of protein stability; Protein chemistry studies; Protein stabilization; Protein aggregation studies; DNA extraction; Primer design; PCR; Site-directed mutagenesis; Cloning; Mammalian cell culture, transfection; Mammalian protein expression and purification. Western blot; Co-immunoprecipitation; Tandem affinity purification (TAP); GST pull-down assay; TEM sample preparation; C. elegans extract preparation.