Andrology-Open Access
Open Access
ISSN: 2167-0250
ISSN: 2167-0250
Department of Urology, Sun Yat-Sen University, Zhuhai, China
Research Article
Crosstalk between FAP+ Fibroblasts and Tumor-Associated Macrophages in Prostate Cancer Unveiled by Single-Cell Sequencing and Spatial Transcriptomics
Author(s): Xinyu Li*, Fei Zheng, Zepeng Dong, Hanchao Liu and Tingting Wu*
Background: Prostate Cancer (PCa) has a wide range of diversity and uncertainty in tumor progression. Despite the availability of multidisciplinary therapy, the mortality of patients with advanced PCa still remains high. In-depth research of the intercellular communications in the Tumor Microenvironment (TME) will improve our understanding of the tumorigenesis mechanism of PCa and bring up potential therapeutic targets to patients with PCa.
Methods: We integrate data including bulk RNA sequencing, single-cell RNA sequencing and spatial transcriptomics to investigate the intercellular communications between FAP+fibroblasts and tumor-associated macrophages in PCa. Immumohistochemical and immunofluorescence staining were performed for verification of the analysis.
Results: We analyzed single-cell sequencing data encom.. View More»