Journal of Medical Diagnostic Methods
Open Access

Laboratory of Pathology and Development, Institute for Quantitative Biosciences, The University of Tokyo, Bunkyo City, Tokyo, Japan

Publications

• Research Article   
  Reactive Blue Dye: Highlights of Vacuoles in Human Sperm
  Author(s): Satoru Kaneko*, Yuki Okada, Satoshi Yokota and Kiyoshi Takamatsu
  
  Objectives: The WHO reference manual for andrology considers the outline of sperm head as a key parameter for assessing male infertility and classifies internal vacuoles as head defects. The features of vacuoles were heterogeneous among interindividual as well as inter-sperm. The present study reported some unique properties of Reactive Blue 2 (RB2). The dye exhibited pH-dependent cellular specificity for human sperm and spermatid. Materials and Methods: Human sperm and spermatid were stained with RB2 at pH 10.0. Results: RB2 stained human sperm and spermatids, which appeared as translucent bluish bodies, but did not stain lymphocytes, while inner vacuoles appeared as toneless spots. RB2 staining also revealed degraded spermatids that had undergone protamination but were arrested prior to tail elongation. The majority.. View More»
  DOI: 10.35248/2168-9784.23.12.400

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