Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

Xiaogang Wu

Nevada Institute of Personalized Medicine, University of Nevada, Las Vegas, Nevada 89054-4004, United States of America

  • Research Article   
    New Minimotifs and their Functions in the C-Terminome
    Author(s): Surbhi Sharma, Nemanja Novakovic, Zachary Thomas FitzHugh, Alexandria Bragg, Stephen Brooks, Xiaogang Wu and Martin R. Schiller*

    Several protein domains and receptors recognize minimotifs on the short carboxyl termini of proteins and control protein clustering, trafficking, and posttranslational modifications. These functional C-terminal minimotifs are found on approximately 17% of proteins in the human proteome, with 83% of unknown functional significance. We tested a bioinformatic/proteomic approach to systematically identify new C-terminal minimotif functions. We selected 30 putative consensus C-terminal minimotifs based on their fold-enrichment and sequence complexity. These 30 consensus minimotifs had instances on 16% of the C-termini in the human proteome. Binding partners for a representative instance for each consensus C-terminal minimotif were identified by affinity purification liquid chromatography-tandem mass spectrometry. We validated the approach with a PDZ binding C-termin.. View More»

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