Immunotherapy: Open Access
Open Access
ISSN: 2471-9552
ISSN: 2471-9552
Department of Economic and Technological Development Zone, Daxing District, Beijing, People's Republic of China
Research Article
Development and Characterization of Neutralizing Antibodies against SARS-CoV-2
Author(s): Yao Zhang*, Ling Li*, Yakun Li, Zengmin Yang, Aijing Zhang, Ercui Shen, Yun Liu, Yan Wang, Minghui Zheng, Xiao Chen, Shuming Wu, Haijiang Zhang* and Yongjiang Liu*
Background: The novel coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 that belongs to
betacoronaviruseis, and is an enveloped, single and positive-stranded RNA virus. In this study, we describe the
production and characterization of four monoclonal antibodies with neutralization activities.
Methods: Thirteen monoclonal antibodies that identified positive were characterized by neutralization assays,
binding affinities identification, and the indirect sandwich Enzyme-Linked Immunosorbent Assay (ELISA) to
evaluate the immunocompetence. The genes of neutralizing antibodies were sequenced and analyzed.
Results: All monoclonal antibodies recognized two different epitopes respectively, including the receptor binding
domain and human fragment crystallizable, and the binding affinities are also different.. View More»
DOI:
10.35248/2471-9552.23.09.231