Journal of Medical Diagnostic Methods
Open Access
ISSN: 2168-9784
+44 1300 500008
ISSN: 2168-9784
+44 1300 500008
Mulago National Referral Hospital, Kampala, Uganda
Research Article
Diagnostic performance of a multiplex PCR in identifying pneumonia micro-organisms in sputum of HIV positive patients in Uganda
Author(s): Daniel Orit*, William Worodria, Alfred Andama, Henry Kajumbula, Emmanuel Mande and Richard Kwizera
Background: Pneumonia remains a frequent cause of morbidity and mortality among HIV infected people in sub-
Saharan Africa. A disease with such a burden requires an appropriate diagnostic modality to allow a logical approach
to guide treatment. Conventional diagnostic methods although available are not fully utilized due to associated
diagnostic limitations, and as such present challenges in the successful management of pneumonia. It is therefore
necessary to evaluate a multiplex Polymerase Chain Reaction (PCR) assay that is rapid, sensitive and specific in
detection of pneumonia microorganisms hence improving time to antimicrobial therapy.
Objective: We aimed to compare the performance of the Bio Fire® Film Array® pneumonia multiplex PCR panel
against a composite reference standard.
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