Journal of Medical Diagnostic Methods
Open Access

Translational research laboratory, Infectious Diseases Institute, College of Health Sciences, Makerere University, Kampala, Uganda

Publications

• Research Article   
  Diagnostic performance of a multiplex PCR in identifying pneumonia micro-organisms in sputum of HIV positive patients in Uganda
  Author(s): Daniel Orit*, William Worodria, Alfred Andama, Henry Kajumbula, Emmanuel Mande and Richard Kwizera
  
  Background: Pneumonia remains a frequent cause of morbidity and mortality among HIV infected people in sub- Saharan Africa. A disease with such a burden requires an appropriate diagnostic modality to allow a logical approach to guide treatment. Conventional diagnostic methods although available are not fully utilized due to associated diagnostic limitations, and as such present challenges in the successful management of pneumonia. It is therefore necessary to evaluate a multiplex Polymerase Chain Reaction (PCR) assay that is rapid, sensitive and specific in detection of pneumonia microorganisms hence improving time to antimicrobial therapy. Objective: We aimed to compare the performance of the Bio Fire® Film Array® pneumonia multiplex PCR panel against a composite reference standard. .. View More»
  

  Abstract PDF