Immunological Disorders and Immunotherapy

Immunological Disorders and Immunotherapy
Open Access

ISSN: 2593-8509

Tiejian Zhao

Department of Physiology, Guangxi University of Chinese Medicine, Nanning, Guangxi, China

  • Research Article   
    Curcumol Targets Gls1 to Regulate Glutamine Metabolism and Induce Senescence of Hepatic Stellate Cells to Exert Anti-Hepatic Fibrosis Mechanism
    Author(s): Xuelin Duan*, Tiejian Zhao, Jiaru Wang, Jiahui Wang and Yang Zheng

    Objective: Curcuma has long been used as a Chinese herbal medicine for the treatment of chronic liver disease, and curcumol is its main active ingredient. This study explores the molecular mechanism of curcumol regulating glutamine metabolism, hoping to provide new ideas for the prevention and treatment of chronic liver disease. Methods: We used LPS to activate hepatic stellate cells. The effects of curcumol on glutamine metabolism and cell proliferation were detected by kit. The effects of curcumol on GLS1, senescence-related proteins and extracellular matrix expression were observed by Western blotting and RT-PCR. The effect of curcumol on cell cycle was detected by flow cytometry. Furthermore, after overexpression of GLS1, we observed the effects of curcumol on glutamine metabolism, expression of related molecules and cell cycle. .. View More»
    DOI: 10.35248/2593-8509.23.8.135

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