Journal of Hematology & Thromboembolic Diseases
Open Access
ISSN: 2329-8790
ISSN: 2329-8790
Department of Hematology, Pakistan Thalassemia Welfare Society (PATHWEL), Rawalpindi, Pakistan
Research
FLAER Based Paroxysmal Nocturnal Hemoglobinuria Clone Detection in Patients of Aplastic Anemia: Single Centre Experience from a Developing Country
Author(s): Zohra Farooq*, Ghassan Umair Shamshad, Mehreen Ali Khan, Muhammad Yousaf and Sobia Umar
Background: Paroxysmal Nocturnal Hemoglobinuria (PNH) is a rare acquired genetic hematopoietic stem cell disorder due to a somatic mutation of the X-linked Phosphatidyl-Inositol Glycan (PIG-A) gene. It is closely related to aplastic anemia with a significant number of aplasia patients having a PNH clone.
Methodology: A total of 115 aplastic anemia patients were enrolled in the study. PNH testing was done by multiparameter flow cytometry analysis specifically using Fluorescein-Labeled Proaerolysin (FLAER) to increase the sensitivity of the procedure. The antibody panel for neutrophils and monocytes consisted of CD45, CD15, CD157 and CD64 along with FLAER. Whereas, CD235a was used for gating the true RBCs population on which the co expression of CD59 was observed. This population was differentiated into RBCs with complete and partial deficienc.. View More»
DOI:
10.35248/2329-8790.23.11.536