Journal of Leukemia
Open Access
ISSN: 2329-6917
+44 1300 500008
ISSN: 2329-6917
+44 1300 500008
Department of Medical Oncology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India
Research Article
Proposed Single-Tube Ten-Color Antibody Panel to Optimize Resources for Measurable Residual Disease Detection in B-Acute Lymphoblastic
Leukemia Based on Leukemia Associated Immunophenotype Evaluation at Diagnosis: A Single Center Experience from Southern India
Author(s): Sujaya Mazumder, Prabhu Manivannan*, Chandni Bhandary, Rakhee Kar, Smita Kayal and Debdatta Basu
Purpose: The importance of Measurable Residual Disease (MRD) detection in B-acute lymphoblastic leukemias (BALL), has led to identifying ways to increase the sensitivity of detecting MRD by multicolor flow cytometry (MFC) and distinguishing it from the hematogones. The identification of cost-effective panel by MFC is necessary and hence this study was undertaken with an objective to propose a presumptive single tube ten-color antibodies panel for MRD detection by the analysis of immunophenotypic profile of BALL with respect to seven newer Leukemia-Associated Immunophenotype (LAIP) markers at diagnosis namely CD9, CD44, CD58, CD73, CD81, CD86 and CD123, in addition to six backbone markers (CD45, CD34, CD38, CD10, CD19 and CD20) which would be useful in a resource constrained setting.
Methods: This was a retrospective, cross-sectional study. A.. View More»
DOI:
10.35248/2329-6917.22.10.308