ISSN: 2471-9315
Department of Biomedical and Diagnostic Services, University of Tennessee, Tennessee, USA
Research Article
Comparison of Multiplex Real-Time PCR with Standard Urine Culture for Identification of Pathogens Associated with Canine Urinary Tract Infections
Author(s): Samantha J Guida*, Joseph Bartges, Rebekah Jones, Caleb Young and Maria Cekanova
Conventional aerobic microbial urine culture with antimicrobial susceptibility testing (UCS) is reference standard for diagnosis of canine Bacterial Urinary Tract Infections (BUTIs). Emerging multiplex real-time PCR (qPCR) may be a useful diagnostic test when performed in conjunction with UCS. The aim of this study was to compare qPCR with UCS for identification of uropathogens in canine urine. Twenty-three frozen isolates of canine uropathogens were selected from an archive and grown on blood agar plates. Following growth on blood agar, colonies from each isolate were inoculated into sterile canine urine, creating 23 positive contrived urine specimens. Urine specimens were incubated for 40 hours at 38°C. Samples were split into two sets of 23 specimens; the first set was analyzed by UCS, and the second set was analyzed by qPCR. Two specimens of sterile urine were used as negativ.. View More»
DOI:
10.35248/2471-9315.23.9.253