Enzyme Engineering
Open Access

Department of Bioscience and Biotechnology, Institute of Allied Medical Science and Technology, NIMS University, Rajasthan, India

Publications

• Research Article   
  Therapeutic Effectiveness of L-Methioninase as Anticancer Agent: In silico versus In vivo analysis
  Author(s): Bhawana Kharayat and Priyanka Singh*
  
  L-methioninase has significant application as chemotherapeutic anticancer drug in pharmaceutical sector. The catalytic activity of L-methioninase has depleted the exogenous supply of L-methionine by arresting growth of methionine dependent cancer cells. In this study, L-methioninase was purified from fermented broth of new bacterial isolate Pseudomonas stutzeri MTCC 101 with molecular weight (45 kDa), Michaelis constant (Km=36.5 mM) and maximum activity (Vmax=500 μmole/min/mg protein). This enzyme was resolved into 291 peptide sequences after MALDI-TOF/MS spectroscopy. Molecular docking and simulation studies as in silico approach had confirmed its higher binding affinity towards L-threonine and L-methionine substrates. The substrate specificity test had confirmed its maximum activity for specific amino acid L-methionine substrate. The high c.. View More»
  DOI: 10.35248/2329-6674.24.13.234

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