ISSN: 2471-9315
Department of Biological Sciences, Ajayi Crowther University, Ibadan, Nigeria
Research
Fatty Acid Methyl Ester Analysis of Olive Oil Degraded by Candida parapsilosis
Author(s): Popoola Bukola Margaret* and Onilude Anthony Abiodun
Fatty acids are the major components of lipids; and the physical, chemical and physiological properties of a lipid class depend primarily on its fatty acid composition. Gas chromatography method can be used for the identification of microbiological degraded fatty acids in vegetable oils as methyl ester. The use of GC for FAME Quantification determination with flame ionization detectors has been effectively used for some time. It is highly sensitive, high accuracy and high reproducibility. It has advantages over Mass Spectrometry (MS) when analyzing n-6 and n-3 fatty acid metabolism as it is able to distinguish between structurally similar fatty acids as it uses retention time for labeling as opposed to atomic mass. MS is able to identify fatty acids within a sample but unable to distinguish double bond positions in stereoisomers and therefore unable to tell certain fatty acids apart... View More»
DOI:
10.35248/2471-9315.20.6.168