Applied Microbiology: Open Access
Open Access

Department of Molecular Medicine, National Institute of Tuberculosis and Respiratory Diseases, New Delhi, India

Publications

• Research Article   
  Rapid and Accurate Point of Care Diagnosis Tool to Combat an Ancient Foe
  Author(s): Neha Sharma, Paras Singh*, Monika Malik, Sangeeta Sharma, Khalid U. Khayyam, Ravindra Kumar Dewan and Neeraj Kumar
  
  Tuberculosis (TB) is known to cause the highest number of infection related deaths worldwide. In 2016, WHO approved TB-LAMP (Loop-Mediated Isothermal Amplification) assay as a replacement for smear microscopy for the diagnosis of PTB in adult TB suspects. However, more epidemiological research should be conducted to support the deployment of the TB-LAMP program in peripheral level healthcare settings. This study analyzed the diagnostic efficacy of rapid and inexpensive TB-LAMP assay for the diagnosis of PTB. TB-LAMP assay displayed 1.8-fold (minimum 49.2%) higher positivity rates than the smear microscopy test (maximum 29.6%). In comparison to the composite reference standard, TB-LAMP assay was observed to be 84.3% sensitive and 96.8% specific for the diagnosis of PTB. The positive predictive value and negative predictive value of the TB-LAMP assay were 88.2 (95%CI: 77.3-94.3) and 95... View More»
  DOI: 10.35248/2471-9315.23.9.268

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