Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol

Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access

ISSN: 2375-4508

+44 1478 350008

Noureddine Louanjli

LABOMAC Laboratory of Clinical Analysis and IRIFIV Fertility Center, AFC Fertility Center - Executive Vice President of the (IRIFIVAISRG) Casablanca, Morocco

Publications
  • Research   
    Mineral Oil Covering on Early Human Embryo Culture: Comparative Analysis of Oil Embryo Culture Incubation
    Author(s): Noureddine Louanjli

    The oil overlay in micro drop culture systems prevents medium evaporation, helps to maintain appropriate pH and osmotic conditions and protects from microbial contamination. In the present study, we prospectively compared covering by Ovoil™, a paraffin oil, and LiteOil, a mineral oil, on the in vitro development of human embryos and their suitability for transfer/freezing at day 3 and live birth rate. One hundred and one patients undergoing in vitro fertilization (IVF) treatment by intracytoplasmic sperm injection (ICSI) were enrolled in our study. After ICSI, 1237 oocytes were 1:1 randomly allocated into 2 groups according to the type of overlaying oil: Ovoil™ (616 oocytes) or LiteOil (621 oocytes). Fertilization rate was assessed around 18 hour’s post-insemination (hpi) and embryos were checked for early cleavage at 25 hpi. Embryo morphology was recorded on days 2 .. View More»

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