International Journal of Physical Medicine & Rehabilitation
Open Access
ISSN: 2329-9096
ISSN: 2329-9096
Evotec SE, 22419 Hamburg, Germany
Research Article
Transcriptome Changes and Neuronal Degeneration in an In Vitro Model of B Vitamin Depletion
Author(s): Niels Banek, Leonie Martens, Natalie Daluege, Nikisha Carty, Sebastian Schmeier, Oltea Trutz, Kenneth W. Young* and Patrizia Bohnhorst*
Background: The neurotropic B vitamins such as thiamine (B1), pyridoxine (B6), and cobalamin (B12) play a pivotal role in the maintenance of neuronal viability and contribute essentially to a healthy nervous system. Furthermore, it is well recognised that deficiencies in neurotropic B vitamins can lead to a variety of neurological conditions and peripheral neuropathies. However, to date, the effect of B vitamin depletion on modulation of genes and cellular pathways has not been fully assessed.
Materials and Methods: In this current study we used RNA sequencing and transcriptomic analysis to investigate gene expression changes in primary cultures of mouse Dorsal Root Ganglion (mDRG) neurons in response to neurotropic B vitamin depletion. Gene Ontology (GO) enrichment analysis identified significant changes in pathways and processes associated.. View More»
DOI:
10.35248/2329-9096.22.S18.002