Internal Medicine: Open Access

Internal Medicine: Open Access
Open Access

ISSN: 2165-8048

Ndogo Eteme Olivier

Department of Organic Chemistry, University of Yaounde I, Cameroon, South Africa

  • Review Article   
    Discovery of Novel Flavonoids Fractions: Based Enzymes Inhibitors of Prolyl Oligopeptidase
    Author(s): Ndogo Eteme Olivier*

    The following work describes the purification of recombinant expressed Prolyl Oligopeptidaseby GSH affinity capture beads via a GST-tag and protease cleavage site. The purification steps were monitored with Commission stained protein gel analysis. Therefore, an enzyme inhibition assay design for a well-established internally quenched fluorophore as substrate was established. Ethylacetate (flavonoid extract), hexane and dichloromethane plant extracts of different species of Allexis were analyzed for inhibition on POP... View More»

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