The following work describes the purification of recombinant expressed Prolyl Oligopeptidaseby GSH affinity capture beads via a GST-tag and protease cleavage site. The purification steps were monitored with Commission stained protein gel analysis. Therefore, an enzyme inhibition assay design for a well-established internally quenched fluorophore as substrate was established. Ethylacetate (flavonoid extract), hexane and dichloromethane plant extracts of different species of Allexis were analyzed for inhibition on POP.
Published Date: 2021-12-29; Received Date: 2021-12-08