Journal of Hepatology and Gastrointestinal disorders
Open Access
ISSN: 2475-3181
ISSN: 2475-3181
Department of Pathology, University of Colombo, Colombo, Sri Lanka
Research Article
Human Epidermal Growth Factor Receptor-2(HER-2) Expression in Sri Lankan Gastric Adenocarcinoma Patients: Analysis of the Concordance of HER2 Expression and Survival Based on Silver In-Situ Hybridization (SISH)
Author(s): Duminda Subasinghe*, Nathan Acott, Pasyodun Koralage Buddhika Mahesh, Sivasuriya Sivaganesh, Ananthi Samarasinghe, Mariyan Priyanthi Kumarasinghe, Dharmabandhu Nandadeva Samarasekera and Menaka Dilani Samarwickrema Lokuhetty
Background: Accurate HER2 status is crucial for Gastric Adenocarcinoma (GC) patient selection for antiHER2
therapy. It is assessed Immunohistochemically (IHC) for protein expression, and by Silver In-Situ Hybridization
(SISH) for gene copy number. This study aimed to evaluate the concordance of HER2 status by IHC/SISH analyses
and HER2-SISH based survival.
Methods: This prospective study includes 145 GC’s (excluding gastro-oesophageal-junction tumours) from the
National Hospital of Sri Lanka with defined demographic, clinical-radiological-pathological characteristics. HER2-
IHC was assessed by DAKO A0485, RealTM Envision system and interpreted using Ruschoff criteria. HER2-SISH
was assessed with INFORM HER2 dual ISH DNA Probe Cocktail. Concordance between HER2 IHC/SISH results
was determined by Cohen’s kappa st.. View More»