Immunome Research

Immunome Research
Open Access

ISSN: 1745-7580

+32 28087017

Masakazu Tsuchiya

Charles River, Microbial Solutions, 1023 Wappoo Road, Suite 43B, Charleston, SC 29407, USA

  • Original Research Article   
    Mechanism of Low Endotoxin Recovery Caused by a Solution Containing a Chelating Agent and a Detergent
    Author(s): Masakazu Tsuchiya*

    Mechanism of Low Endotoxin Recovery (LER) was investigated by observing the change in particle sizes and activity of endotoxin in LER solutions containing sodium citrate and polysorbate 20. Interestingly, endotoxin aggregates were not dispersed to be smaller particles under LER conditions according to the decease of the activity. This observation was different from previous predictions of the mechanism. Endotoxin aggregates were observed by Dynamic Light Scattering, and activity was measured by the Limulus amebocyte lysate test. Particles with sizes similar to endotoxin aggregates were always observed despite different remaining activity of spiked endotoxin. This observation differed from previously proposed mechanisms for LER that dispersed endotoxin aggregates were smaller in sizes. Based on the findings, a new mechanism of LER is proposed. Purified endotoxin forms non-lamellar cubi.. Read More»
    DOI: 10.35248/1745-7580.19.15.166

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