Biochemistry & Pharmacology: Open Access
Open Access
ISSN: 2167-0501
ISSN: 2167-0501
Department of Large Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Japan
Research Article
Carboxylic Acids with Certain Molecular Structures Decrease Osmotic Fragility against Osmotic Pressure in Cattle Erythrocytes In Vitro: Appearance of a Wedge-like Effect Similar to RBCs in Other Animal Species
Author(s): Hitoshi Mineo* and Masaharu Moriyoshi
Osmotic fragility (OF) in red blood cells (RBCs) is a useful tool for evaluating the actions of various chemicals on the cell membrane in vitro. The effects of monocarboxylic and dicarboxylic acids on OF were evaluated in cattle RBCs. Isolated cattle RBCs were immersed in various carboxylic acids at 0-100 mM in a buffer solution for 1 hr and the 50% hemolysis was then determined by soaking in 0.1-0.8% NaCl solution. Although n-caprylic acid at 100 mM induced hemolysis, the other monocarboxylic acids possessing straight hydrocarbons did not affect OF. The dicarboxylic acids possessing straight hydrocarbons, except for glutaric acid, decreased OF in a dose-dependent manner. Some monocarboxylic acids with branched hydrocarbons tended to decrease OF, but these changes were not statistically significant. Although cyclopentanecarboxylic and cyclohexanecarboxylic acids at 100 mM dec.. View More»
DOI:
10.35248/2167-0501.19.8.264