Journal of Glycobiology
Open Access
ISSN: 2168-958X
ISSN: 2168-958X
Department of Biology, College of Liberal Arts and Science, The University of Iowa, Iowa 430015, USA
Research Article
Increased Sialylation of N- and O-Glycans on Serum Maackia amurensis Lectin II Binding Glycoproteins in Autistic Spectrum Disorder
Author(s): Yannan Qin, Yanni Chen*, Ganglong Yang, Lingyu Zhao, Zhuoyue Shi and Chen Huang*
Background: The pathophysiology of Autistic Spectrum Disorder (ASD) is not fully understood and there are no diagnostic or predictive biomarkers. In our previous study we found that expression of serum Siaα2-3 Gal/ GalNAc recognized by Maackia amurensis Lectin II (MAL-II) as well as MAL-II Binding Glycoproteins (MBGs) were significantly increased in ASD compared to Typically Developing (TD) children; however the specific glycoforms of MBGs remain unclear.
Method: In this study, N- and O-glycans on sera MAL-II binding glycoproteins (MBGs) from 60 children with ASD and 60 age-matched TD children were profiled by using lectin-magnetic particle conjugate assisted MALDI-TOF/ TOF-MS analysis.
Results: A total of 16 representative N-glycans including high-mannose, complex and hybrid, bi-/tri-antennary structures and bisec.. View More»