Journal of Antivirals & Antiretrovirals
Open Access
ISSN: 1948-5964
ISSN: 1948-5964
Dalian Gentalker Bio-Tech Co., Ltd., Jinqi Road No.9, Dalian, China
Research
Detection of a Novel Coronavirus (SARS-CoV-2) By Real-Time Reverse Transcription-Polymerase Chain Reaction, China, 2020
Author(s): Huahua Feng, Honglong Wu, Huagui Wang, Jianying Yuan, Lu Chen, Hui Jin, Lingling Yang, Jinyin Zhao*, Licheng Liu* and Weijun Chen*
Background: During the outbreak of unexplained pneumonia in the city of Wuhan in the late December, 2019, a novel coronavirus named SARS-CoV-2 was identified as the cause of this outbreak.
Methods: A real-time polymerase chain reaction, which targets the orf1ab gene of viral genome, was established to detect and identify the SARS-CoV-2. We used this assay to screen 309 samples from persons with suspected SARS- CoV-2 infection in Wuhan. Then 6 close-phylogenic coronaviruses and 7 viruses which could cause pneumonia were detected. Moreover, 57 clinical samples infected with other viruses and 77 healthy samples were also tested.
Results: The limit of detection of the assay was 6.25 copies per reaction in the detection of cRNA transcribed in vitro. The results of detection of throat and fecal swabs from person.. View More»