Journal of Molecular Imaging & Dynamics
Open Access
ISSN: 2155-9937
ISSN: 2155-9937
Department of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, Tainan, Taiwan
Opinion Article
Microtumor Molecular Imaging with BerEp4: Toward Theranostics for Basal Cell Carcinoma
Author(s): Klussmeier Anja*
The most prevalent cancer in humans, basal cell carcinoma (BCC), appears macroscopically and microscopically identical to many other skin lesions, making differential diagnosis challenging. With the goal of improving the precision and accuracy of diagnosis, we are developing a method for quantitative molecular imaging of BerEP4, a Trans membrane biomarker for BCC. The purpose of this pilot study was to determine the affinity and selectivity of BerEp4 antibody and to see if it could be used to develop theranostic probes for BCC. We show that a film/pellet phantom can be used to recover individual signal increases using our photon-counting fluorescence macro detection method. Furthermore, we demonstrate that a two-photon stimulated fluorescence/backscatter confocal imaging system can view the BerEP4 antibody/antigen combination on the surface of BerEP4-expressing cancer cells in three d.. View More»