Advanced Techniques in Biology & Medicine
Open Access
ISSN: 2379-1764
ISSN: 2379-1764
Dr, Drug Discovery and Development Center, Office of Advanced Science and Technology, Thammasat University (Rangsit Campus), Pathumthani, 12120, Thailand
Research Article
High-throughput Bioassay and HPLC-UV Methods for Determination of the Bioactivity of Atractylodes lancea and Its Bioactive Constituent Atracylodin in Biological Samples: Application for Pharmacokinetic Study in Patients with Advanced-stage Cholangiocarcinoma
Author(s): Anurak Cheoymang, Nadda Muhamad, Inthuon Kulama and Kesara Na-Bangchang*
The study aimed to establish a high-throughput bioassay method for the determination of total bioactivity of Atractylodes lancea (AL) in human serum samples. In addition, a simple HPLC-UV method for the determination of plasma concentrations of Atractylodin (ATD), the main bioactive component of AL, was also developed. For the bioassay method, Staphylococcus aureus (S. aureus) ATCC 25923 strain was used as a test organism. Inhibition of bacterial growth was assessed using MTT assay. The calibration curve was prepared from the concentration response curve in serum (0, 0.39, 0.78, 1.56, 3.13, 2.56, and 50 ng/ µl), which was linear with correlation coefficients better than 0.990. The Limit of Quantification (LOQ) was 1.66 µg/ml using 20 µl serum samples. The HPLC-UV assay procedure was developed based on reversed-phase chromatography using Hyp.. View More»
DOI:
10.35248/2379-1764.22.10.343