Potential Growth Enhancement of Crops Using Pure Culture of Rhizobium Spp.
Author(s): Josiah Asime Lennox
Background: The quest for increased food production for ever-increasing population growth is a continuous venture. This has necessitated this research to use cheaply available resources, microorganisms in the production of biofertilizer. Methods: A soil sample was obtained from a potato farm and a ten-fold dilution prepared up to 10-8. One milliliter from dilutions 10-4, 10-5 and 10-6 was pipetted and transferred into 3 Petri dishes labeled accordingly. Molten Rhizobium agar medium was carefully poured into the plates, swirled gently to mix and incubated at 37oC for 24 hours. The discrete colonies were characterized. The broth culture of these colonies were prepared in nutrient agar. Seeds such as corn, beans, groundnut, and pumpkin were treated with broth cultures and planted in soil contained in plastic buckets. These were observed for 14 day.. Read More»