Journal of Plant Biochemistry & Physiology
Open Access

Plant Biochemistry Laboratory, Department of Biology, Concordia University, 7141 Sherbrooke Street West, Montréal, Québec, H4B 1R6, Canada

Biography

Jian-Min Zhou currently works at the Center for Genome Biology, the Chinese Academy of Sciences. Jian-Min does research in Botany, Genetics, and Immunology. Their most recent publication is 'Roles of receptor-like cytoplasmic kinase VII members in pattern-triggered immune signaling'. His study interests are major in Molecular Biology, Botany, Immunology, Genetics, RNA, Gene Expression, Pathogens, etc. He published nearly about 174 articles in many journals and the articles are informative and got good citations. He had worked on 1 project on redox homeostasis and stress responses in animals and plants which is much informative and interesting.

Publications

• Research Article   
  Tobacco Ntomt2 Revisited: Multiple O-Methylation of Quercetin is Catalyzed by a Single Gene Product
  Author(s): Jian-Min Zhou, Amira Moheb and Ragai K Ibrahim*
  
  Tobacco NtOMT2 was previously reported as an inducible, o-diphenol O-methyltransferase (OMT) that exhibits similar preferences towards quercetin and caffeic acid as substrates. In this report, we further examine the substrate specificity of  NtOMT2 based on enzyme assays, kinetic data and phylogenetic analysis. We demonstrate that NtOMT2 is a flavonol OMT that catalyzes multiple O-methylation of the flavonol quercetin giving rise to its mono-, di- and trimethyl ether derivatives. Mono-methylation of quercetin on the 3-, 3′-/4′- and 7-hydroxyl groups occurs in a random fashion according to the physio-chemical properties of their hydroxyl groups, together with their similar binding energies in the NtOMT2 binding pocket. Homology modeling of NtOMT2 with the Medicago sativa COMT as a template indicates that the H-bonding network between the functional hydrox.. View More»
  DOI: 10.35248/2090-7214.13.1.107

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