ISSN: 2471-9315
Department of Laboratoire d'Agro Biotechnologie et de Nutrition des Zones Semi Arides, Université de Tiaret, Tiaret, Algeria
Research Article
Description of a Clinical Strain of Mycobacterium cambodiensis sp. nov., a New Member of the Mycobacterium simiae Complex
Author(s): Fatah Tazerart, Jamal Saad, Muriel Militello, Sophie Alexandra Baron, Michel Drancourt* and Sylvain Godreuil
A bronchoalveolar lavage sample was collected by bronchoscopy from a 25-year-old Cambodian male patient with suspected clinical tuberculosis and was inoculated in Löwenstein-Jensen medium. Colonies of a rapidly growing, non-chromogenic Gram-positive and acid-fast bacterium were investigated. Scanning electron microscopy showed 1.2 ± 0.29 μm-long and 0.58 ± 0.07 μm-large bacilli that could not be identified using routine matrix-assisted laser desorption ionization-time of flight-mass spectrometry and phenotypic tests (API® ZYM, API® Coryne and Biolog® Phenotype MicroArray assays). In vitro, the isolate was susceptible to isoniazid, amikacin and trimethoprim- sulfamethoxazole. Whole-genome sequencing yielded a 5,703,981-bp draft genome that displayed 69.3% of GC content with 5,207 coding-protein genes and 56 predicted.. View More»
DOI:
10.35248/2471-9315.21.7.211